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                              May 5, 2023

       Ann Marie Sastry
       Chief Executive Officer
       Amesite Inc.
       607 Shelby Street, Suite 700 PMB 214
       Detroit, MI 48226

                                                        Re: Amesite Inc.
                                                            Amendment No. 1 to
Form S-1
                                                            Filed April 28,
2023
                                                            File No. 333-270512

       Dear Ann Marie Sastry:

             We have reviewed your amended registration statement and have the
following
       comments. In some of our comments, we may ask you to provide us with
information so we
       may better understand your disclosure.

              Please respond to this letter by amending your registration
statement and providing the
       requested information. If you do not believe our comments apply to your
facts and
       circumstances or do not believe an amendment is appropriate, please tell
us why in your
       response.

              After reviewing any amendment to your registration statement and
the information you
       provide in response to these comments, we may have additional comments.
Unless we note
       otherwise, our references to prior comments are to comments in our March
27, 2023 letter.

       Amendment No. 1 to Form S-1 filed April 28, 2023

       Selling Stockholders, page 12

   1.                                                   We note your revised
disclosure in response to prior Comment One. FINRA's broker-
                                                        dealer database
identifies Mr. Garlikov as a broker-dealer. Please confirm the status of
                                                        Mr. Garlikov and revise
the disclosure accordingly.
       Incorporate of Documents by Reference, page 20

   2.                                                   We note your revised
disclosure in response to prior Comment Two and reissue our
                                                        comment in part. Please
revise to specifically incorporate by reference your current report
                                                        on Form 8-K filed March
10, 2023.
 Ann Marie Sastry
Amesite Inc.
May 5, 2023
Page 2

       Please contact Mariam Mansaray, Staff Attorney at 202-551-6356 or
Matthew Crispino,
Staff Attorney at 202-551-3456 with any other questions.



                                                        Sincerely,
FirstName LastNameAnn Marie Sastry
                                                        Division of Corporation
Finance
Comapany NameAmesite Inc.
                                                        Office of Technology
May 5, 2023 Page 2
cc:       Richard A. Friedman, Esq.
FirstName LastName