November 7, 2024 Ann Marie Sastry, Ph.D. Chief Executive Officer Amesite Inc. 607 Shelby Street Suite 700 PMB 214 Detroit, MI 48226 Re: Amesite Inc. Registration Statement on Form S-3 Filed November 5, 2024 File No. 333-282999 Dear Ann Marie Sastry Ph.D.: This is to advise you that we have not reviewed and will not review your registration statement. Please refer to Rules 460 and 461 regarding requests for acceleration. We remind you that the company and its management are responsible for the accuracy and adequacy of their disclosures, notwithstanding any review, comments, action or absence of action by the staff. Please contact Alexandra Barone at 202-551-8816 with any questions. Sincerely, Division of Corporation Finance Office of Technology cc: Richard A. Friedman, Esq.