FORM 3
 UNITED STATES SECURITIES AND EXCHANGE COMMISSION
Washington, D.C. 20549

INITIAL STATEMENT OF BENEFICIAL OWNERSHIP OF SECURITIES

Filed pursuant to Section 16(a) of the Securities Exchange Act of 1934 or Section 30(h) of the Investment Company Act of 1940
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(Print or Type Responses)
1. Name and Address of Reporting Person *
  Barkett Anthony M.
 2. Date of Event Requiring Statement (Month/Day/Year)
09/25/2020		 3. Issuer Name and Ticker or Trading Symbol
Amesite Inc. [AMST]
(Last)
(First)
(Middle)
C/O AMESITE INC., 607 SHELBY STREET, SUITE 700 PMB 214		 4. Relationship of Reporting Person(s) to Issuer
(Check all applicable)
__X__ Director _____ 10% Owner
_____ Officer (give title below) _____ Other (specify below)
5. If Amendment, Date Original Filed(Month/Day/Year)
(Street)

DETROIT, MI 48226		 6. Individual or Joint/Group Filing(Check Applicable Line)
_X_ Form filed by One Reporting Person
___ Form filed by More than One Reporting Person
(City)
(State)
(Zip)
Table I - Non-Derivative Securities Beneficially Owned
1.Title of Security
(Instr. 4)		 2. Amount of Securities Beneficially Owned
(Instr. 4)		 3. Ownership Form: Direct (D) or Indirect (I)
(Instr. 5)		 4. Nature of Indirect Beneficial Ownership
(Instr. 5)
Common Stock 50,000
D
 

Reminder: Report on a separate line for each class of securities beneficially owned directly or indirectly. SEC 1473 (7-02)
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Table II - Derivative Securities Beneficially Owned (e.g., puts, calls, warrants, options, convertible securities)
1. Title of Derivative Security
(Instr. 4)		 2. Date Exercisable and Expiration Date
(Month/Day/Year)		 3. Title and Amount of Securities Underlying Derivative Security
(Instr. 4)		 4. Conversion or Exercise Price of Derivative Security 5. Ownership Form of Derivative Security: Direct (D) or Indirect (I)
(Instr. 5)		 6. Nature of Indirect Beneficial Ownership
(Instr. 5)
Date Exercisable Expiration Date Title Amount or Number of Shares
Stock Option (Right to Buy)   (1) 03/29/2028 Common Stock 72,917 $ 1.5 D  
Stock Option (Right to Buy)   (2) 03/31/2030 Common Stock 30,000 $ 2 D  
Stock Option (Right to Buy)   (2) 03/31/2030 Common Stock 75,000 $ 2 D  
Stock Option (Right to Buy)   (3) 05/22/2030 Common Stock 150,000 $ 2 D  

Reporting Owners

Reporting Owner Name / Address Relationships
Director 10% Owner Officer Other
Barkett Anthony M.
C/O AMESITE INC.
607 SHELBY STREET, SUITE 700 PMB 214
DETROIT, MI 48226		   X      

Signatures

/s/ Anthony Barkett, Ph.D. 09/25/2020
**Signature of Reporting Person Date

Explanation of Responses:

* If the form is filed by more than one reporting person, see Instruction 5(b)(v).
** Intentional misstatements or omissions of facts constitute Federal Criminal Violations. See 18 U.S.C. 1001 and 15 U.S.C. 78ff(a).
(1) The stock options are fully vested.
(2) The stock options were granted on March 31, 2020, and vest annually in equal amounts over a period of two (2) years, with 50% of the shares vesting on the one-year anniversary of the date of grant and the remaining 50% shares vesting on the two-year anniversary of the date of grant.
(3) The stock options were granted on May 22, 2020, and vest annually in equal amounts over a period of two (2) years, with 50% of the shares vesting on the one-year anniversary of the date of grant and the remaining 50% shares vesting on the two-year anniversary of the date of grant.

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