Exhibit 99.1

Shareholder Update W I N T E R 2 0 2 1

Forward Looking Statements This presentation may contain “forward - looking statements” within the meaning of Section 27A of the Securities Act of 1933 and Section 21E of the Securities Exchange Act of 1934, as amended. For such forward - looking statements, we claim the protection of the safe harbor for forward - looking statements contained in the Private Securities Litigation Reform Act of 1995. As used below and throughout this presentation, the words “we”, “us” and “our” may refer to Amesite individually or together with one or more partner companies, as dictated by context. Such statements include, but are not limited to, any statements relating to our growth strategy and product development programs and any other statements that are not historical facts. Forward - looking statements are based on management’s current expectations and are subject to risks and uncertainties that could negatively affect our business, operating results, financial condition and stock price. Factors that could cause actual results to differ materially from those currently anticipated include: risks related to our growth strategy; risks relating to the results of research and development activities; our ability to obtain, perform under and maintain financing and strategic agreements and relationships; our dependence on third party suppliers; our ability to attract, integrate, and retain key personnel; the early stage of products under development; our need for and continued access to additional funds; government regulation; patent and intellectual property matters; competition; as well as other risks described in our Securities and Exchange Commission filings. We expressly disclaim any obligation or undertaking to release publicly any updates or revisions to any forward - looking statements contained herein to reflect any change in our expectations or any changes in events, conditions or circumstances on which any such statement is based, except as may be required by law. The information contained herein is intended to be reviewed in its totality, and any stipulations, conditions or provisions that apply to a given piece of information in one part of this presentation should be read as applying mutatis mutandis to every other instance of such information appearing herein. © 2021 Amesite Inc. All Rights Reserved. 2

U p d a te Topics Financial Position & Revenue Recent Customer Acquisitions & Partnerships © 2021 Amesite Inc. All Rights Reserved. 3 Highlights on Talent & Team Structure Activities to Date Supporting Both ESG & DEI 4 - 5 6 - 7 8 - 10 11

F i n a n c i al Position D e b t © 2021 Amesite Inc. All Rights Reserved. 4 Operating Cash Us e d Q 1 2 02 2 $0 $1.2M As o f S e p t em b er 3 0 , 2 0 2 1 Amesite’s Financial Position: • G o o d L i q ui d i t y • N o D e b t • E qu i t y L in e t o R a is e a s N e e d e d , El e cti v e ly >1y Cash Remaining $15.0M Cash Remaining on Equity Line $10.6M Cash On Hand

R e v e n u e 27.7% Q 1 2 0 22 Yo Y R e v e n u e Growth © 2021 Amesite Inc. All Rights Reserved. 5 10X FY2021 Revenue Growth Over FY2020 $1.4M Total Contract Value Since Inception $115K Average Deal Size Since Inception As o f S e p t em b er 3 0 , 2 0 2 1 Building Revenue: • Ke y S t a f f P o s i t i o n s F ill e d • Co m p a n y I s D i v e r s if y in g O u r Cu s to m e r B a s e • La r g e A dd r e ssa b l e M a r k et s i n Fo u r S e c t o r s: Enterprise, Higher Ed, Non Profit and Government

Wins: Co n t e x t WAYNE STATE UNIVERSITY Wayne State University (WSU) is a public research university in Detroit, Michigan. It is Michigan’s third - largest university. WSU provides key education and training to the Greater Detroit Area . According to the World Economic Forum, 150 million new technology jobs will be added to the global economy over the next 5 years . © 2021 Amesite Inc. All Rights Reserved. 6 CITY COLLEGE OF NEW YORK The City College of New York (CCNY) is part of the City University of New York (CUNY), the largest urban university system in the United States . In partnership with Amesite, CCNY will equip historically underserved communities with analytical and operation skills to help them pursue career paths in the rapidly changing urban infrastructure sector. In July 2021, the New York City Department of City Planning announced a $133.7 billion, 10 - year strategy for building and enhancing city infrastructure . MICHIGAN WORKS! SOUTHEAST Michigan Works! Southeast (MWSE) is part of the Michigan Works! Association, a sixteen - agency system . The members of the association offer training, education, business development and access to employment opportunities. MWSE serves nearly 18,000 community members through one - on - one career guidance, upskilling job seekers for current labor trends and much more. According to Statista, upskilling is a $370 billion global market with employers spending approximately $1,300 on each employee annually for continuing education . THE HENRY FORD MUSEUM The Henry Ford Museum (THF) provides unique education experiences based on authentic objects, stories, and lives from America’s traditions of ingenuity, resourcefulness and innovation and hosts over 1.7M visitors annually . According to Statista, the market size of the museum industry in the United States reached $12.7 billion in 2020 . SOURCES: https://www1.nyc.gov/site/planning/about/press - releases/pr - 20210720.page https:// www.statista.com/statistics/738519/workplace - training - spending - per - employee/ https:// www.weforum.org/agenda/2021/02/most - in - demand - jobs - 2021 / https:// www.statista.com/statistics/1174784/museum - industry - market - size - us /

Amesite’s Technology, Business Model and Partnerships Support Scaling Customers & Revenue Amesite & Microsoft A s o f S e p t . 1 0 , 2 0 2 1 , A m es i te i s e q u i p p e d t o re a c h a v a s t n e t w o r k o f professionals t h r o u g h o ff e r i ng o n A zu r e Marketplace. T H E I M P A C T By empowering Amesite and putting their platform on Microsoft Azure, we can approach our partner universities around the country and get these solutions out to people who need upskilling c o u r s e s ." “ Tamer Erzurumlu D i r e c t o r o f P a r t n e r S t r a te g y Ed uc a t i o n a t M i c r o s o f t Am e s i t e ’ s V 4 p la t f o r m is n o w o n M i c r o s o f t ’ s Azure Cloud - enabling scalability , speed, an d be s t - i n - cl a ss i n f r a s t r u ctu r e . o r l e ss I s t h e d e li v e r y t i m e n o w f o r a c u s t o m - branded, enterprise scale system for our customers – offering unparalleled speed. la unch e s o f cu s t o m c o n te n t a r e a v a ila b l e , b e c a u s e A m e s i t e ’ s e a s y - to - use p l a t f o r m a n d flexible business model enable fast, high quality content creation. V4 24h 30d 1 , 000 s 98% of A P I s c a n b e i n te g r a te d w i t h A m e s i te ’ s p l a t f o r m b e c a u s e A m e s i t e u s e s a m o d e r n t e c h stack and is fully modularized. © 2021 Amesite Inc. All Rights Reserved. 7 re t e n t i o n ra t e s a c r o ss a ll p r o d uc t s h a v e b ee n a c h i e v e d – A m e s i t e ’ s c u s t o m e r s d e l i v er learning products that work for their users.

People & Growth In 2021… we p r o m o t e d we w e l c o m e d + 1 2 n e w e m p l o y ee s 6 emp l o yees C O R P O R A TE ADMIN. S A L E S FINANCE E N G I N EE R I N G & CUSTOMER SUCCESS INN O V A T I O N & PRODUCT MA R K E T I N G BOARD OF DIRECTORS CEO CFO © 2021 Amesite Inc. All Rights Reserved. 8

People & Growth We are honored to be among the winners of the following workplace and technical awards, including three national workplace excellence awards . • 2 2 E m p l o yee s i n 1 3 S t a t e s • Strong ability to attract talent • Building bench strengths and partnerships in Sales and Marketing • Aligning technical work to disrupt markets and serve our customers © 2021 Amesite Inc. All Rights Reserved. 9

Th e r e s u l t s w e ’ v e s ee n s o f a r a r e unp a ra ll e l ed , t he technology has made the system scalable and easy to use and we can fully achieve our goals of inspiring that next generation of innovators and inventors.” Patricia Mooradian P r e s i d e nt & C E O , Th e H e n r y F o r d M u s e u m “ We chose to partner with Amesite because they offer the most advanced online learning platform in the market today . The f e e db a c k f r o m o u r s t u d e n t s a n d i n s t r uc t o r s h a s b ee n o v e r w h e l m i n g l y p o si t i v e . ” Workforce upskilling and reskilling is needed more urgently n o w t ha n e v e r, gi v e n t h e e v o l u t io n o f t h e w o r k w o r l d . W e c h o s e Amesite to support our own workforce in their professional d e v e l o p m e n t j o u r n e y b e c a u s e we be li e v e i n t h e p o w e r o f t he i r platform to support the engagement , and ultimately the success of our teams." Shamar Herron E x e cu t i v e D i r e c t o r , M i ch i g a n W o r k s ! S o u t h e a s t “ “ Dr. Farshad Fotouhi D e a n o f E n g i n ee r i n g , W a y n e S t a t e U n i v e r s i t y Customer Reviews Employee Reviews: Surveyed Anonymously by Outside Firm* © 2021 Amesite Inc. All Rights Reserved. 10 of A m e s i te e m p l o y ee s f ee l t h e o r g a n i z a t ion promotes a positive culture. of A m e s i te e m p l o y ee s f ee l t h e co m p a n y appreciates their work contributions. of A m e s i te e m p l o y ee s a r e a w a r e o f t h e organization’s strategic goals. 1 0 0 % *Survey dates: 6/14/21 - 6/28/21. Anonymous survey conducted by the National Association for Business Resources of Amesite employees have the authority to make decisions necessary to do their job well. of A m e s i te e m p l o y ee s b e li e v e t h e s e n i or l e a d e r s care about what they have to say. 100 % of Amesite employees feel they are learning and growing on the job. 100 % 100 % 100 % 100 %

Environmental, Social, & Governance Diversity, Equity, & Inclusion 44% 50 % 2 3 % of ou r le a d e r s (M gr ., D i r . a n d E x e c . ) a r e f e ma l e of ou r e mp l o y ees a r e f e ma l e of ou r e mp l o y ees a r e UR M s of ou r B oa r d M e m b e rs a r e d i v e r s e 0 Net Effective Metric Tons of CO2 Emitted with Carbon Offsets 0 # o f u s e r s w h o s e i n f o r m a t i o n was used for secondary purposes $0 in monetary losses as a result of legal proceedings associated with user privacy 1 0 0 % o f g o v e r n a n c e p o l i c i e s ar e c ur r e n t l y i n p l a c e 3 o u t o f 7 © 2021 Amesite Inc. All Rights Reserved. 11