Exhibit 23.1

CONSENT OF INDEPENDENT REGISTERED PUBLIC ACCOUNTING FIRM

We consent to the incorporation by reference in Registration Statement No. 333-250852 on Form S-8 and Registration Statement No. 333-260666 on Form S-3 of our report dated September 28, 2022, relating to the financial statements of Amesite Inc. appearing in this Annual Report on Form 10-K of Amesite Inc. for the year ended June 30, 2022.