Exhibit 23.1

CONSENT OF INDEPENDENT REGISTERED PUBLIC ACCOUNTING FIRM

We hereby consent to the incorporation by reference in this Registration Statement on Form S-8 (the “Registration Statement”) of our report dated September 30, 2024, relating to the financial statements of Amesite Inc. (the “Company”) as of and for the years ended June 30, 2024, and 2023, which includes an explanatory paragraph relating to the Company’s ability to continue as a going concern, appearing in the Annual Report of the Company for the years ended June 30, 2024, and 2023.

/s/ Turner, Stone & Company, L.L.P.

Dallas, Texas

December 23, 2024

Turner, Stone & Company, L.L.P. Accountants and Consultants 12700 Park Central Drive, Suite 1400 Dallas, Texas, 75251 Telephone: 972-239-1660/Facsimile: 972-219-1665 Toll Free: 877-853-4195 Web site: turnerstone.com